normal human bmsc  (Procell Inc)

Journal: Journal of Materials Science. Materials in Medicine

Article Title: Copper tannic acid coordination nanosheet as a potent in-situ antibiotic sustained-release carrier for chronic osteomyelitis

doi: 10.1007/s10856-025-06979-z

Figure Lengend Snippet: The effect of Van@CuTA on BMSCs and HUVECs. A CCK-8 assay demonstrated no toxicity of Van@CuTA toward BMSCs. B Representative images of alizarin red staining, revealing increased calcium deposition in BMSCs treated with Van@CuTA. C , D Representative images and statistical analysis of HUVECs migration assay demonstrating a significant increase in migrated cells following Van@CuTA treatment. E – G Tube formation assay images and corresponding quantifications of total tube length and node number per view indicated that Van@CuTA significantly promoted HUVECs’ tube formation. (* indicated p < 0.05)

Article Snippet: Primary bone marrow mesenchymal stem cells (BMSCs) and Human Umbilical Vein Endothelial Cells (HUVECs) were obtained from ATCC.

Techniques: CCK-8 Assay, Staining, Migration, Tube Formation Assay

Journal: ACS Biomaterials Science & Engineering

Article Title: Spontaneous Crimping of Gelatin Methacryloyl Nanofibrils Induced by Limited Hydration

doi: 10.1021/acsbiomaterials.5c00828

Figure Lengend Snippet: Cellular integration within fibril networks. SEM images of (a) 3T3 fibroblasts and (b) bone marrow-derived mesenchymal stem cells (BMSCs) cultured on straight and crimped fibrils. Statistic comparison of the crimping degree of the fibrils adjacent to adhered (c) fibroblasts and (d) BMSCs between networks with straight and crimped fibrils. (e) Representative SEM image of crimped fibrils soaked in cell culture media without cells for 48 h. (f) Statistic compassion of the crimping degrees of crimped fibrils in networks with and without cell culture. (g) Schematic diagram and SEM images of the cross-section of a crimped fibril mat cultured with fibroblasts for 48 h, showing cell migration to a depth of approximately 200 μm from the mat surface toward the central region. Arrows denote the cut ends of the fibrils at the cross section. (h) Immunofluorescence images of fibroblasts cultured on crimped fibrils, overlaid with bright-field images. (blue for DAPI, magenta for phalloidin, green for vinculin) (i) SEM images of fibroblasts cultured on straight and crimped fibrils for 7 days. Cells had a rounder shape on crimped fibrils, similar to those observed after 48 h culture. P -values lower than 0.0001 are marked as ****. ( n = 50 in panels c, d, f).

Article Snippet: The cell types used were NIH-3T3 fibroblasts (Bioresource Collection and Research Centre (BCRC), Hsinchu, Taiwan) and bone marrow-derived mesenchymal stem cells (BMSCs) from normal human sources (ATCC PCS-500–012TM, Manassas, VA, USA).

Techniques: Derivative Assay, Cell Culture, Comparison, Migration, Immunofluorescence